Investigational Cellular Therapy Platform

JadiCell™

A patented, investigational, allogeneic mesenchymal cell platform derived from the subepithelial layer of umbilical cord tissue and being developed for severe inflammatory and degenerative pulmonary disease.

Allogeneic Umbilical Cord Tissue-Derived Investigational Regenerative Immunology
Overview

A Distinct Umbilical Cord-Derived MSC Platform

JadiCell™ is a patented, investigational, allogeneic mesenchymal cell (MSC) platform derived from the subepithelial layer of umbilical cord tissue. Unlike many MSC products that are isolated from Wharton's jelly, bone marrow, or adipose tissue, JadiCell is manufactured from a distinct anatomical region of the umbilical cord using proprietary methods described in its patent portfolio.

The platform is being developed by Breathe Biologics for severe inflammatory and degenerative pulmonary diseases. Current clinical development includes Phase 3 evaluation in Acute Respiratory Distress Syndrome (ARDS) and an active Investigational New Drug (IND) application supporting development in Chronic Obstructive Pulmonary Disease (COPD).

Rather than replacing damaged lung tissue through engraftment, JadiCell is designed to exert its biological activity primarily through paracrine signaling, immune modulation, secretion of regenerative mediators, and restoration of tissue homeostasis.

Regulation of dysregulated immune responses
Modulation of inflammatory cytokines
Promotion of endothelial repair
Support of epithelial regeneration
Preservation of alveolar-capillary barrier integrity
Enhancement of tissue recovery following severe inflammatory injury

Published clinical research has demonstrated encouraging safety findings together with biomarker changes consistent with immunomodulatory activity in patients with COVID-19-associated ARDS, although larger confirmatory trials are required to establish efficacy.

Cell Biology

A Distinctive Mesenchymal Stromal Cell Profile

JadiCell™ belongs to the broader family of mesenchymal stromal cells (MSCs) but possesses a distinctive biological profile related to its neonatal tissue source and proprietary isolation methodology.

Cells demonstrate the characteristic spindle-shaped fibroblast morphology typical of MSC populations while expressing surface-marker profiles consistent with mesenchymal cell identity.

MSC Profile

At Least 8 Positive Biomarkers

Positive biomarkers reported within the JadiCell™ mesenchymal stromal cell profile include:

  • CD29
  • CD73
  • CD90
  • CD166
  • SSEA4
  • CD9
  • CD44
  • CD146
  • CD105
Hematopoietic Profile

At Least 8 Negative Biomarkers

Negative biomarkers reported within the hematopoietic profile include:

  • CD45
  • CD34
  • CD14
  • CD79
  • CD106
  • CD86
  • CDS0
  • CD80
  • CD19
  • CD117
  • Stro-1
  • HLA-DR
Additional Negative Characteristic
NANOG

Neonatal Tissue Source

JadiCell™ is derived from the subepithelial region of qualified umbilical cord tissue rather than adult bone marrow or adipose tissue.

Allogeneic Platform

The platform is intended for standardized donor-derived manufacturing rather than individualized patient-specific production.

Dynamic Cellular Activity

As a living cellular therapy, JadiCell™ may alter its secretory profile in response to biological signals within inflammatory microenvironments.

Mechanisms Under Investigation
TNF pathway regulation
Increased soluble TNF Receptor-2 (sTNFR2)
Reduction of pro-inflammatory cytokines
Macrophage polarization
Endothelial stabilization
Alveolar epithelial repair
Extracellular vesicle and exosome signaling
Regulation of fibrosis-associated pathways

Scientific qualification: These mechanisms remain areas of active investigation and should not be interpreted as established clinical effects of JadiCell™.

Differentiation

Multipotent Cellular Characteristics

JadiCell demonstrates the multipotent differentiation characteristics expected of mesenchymal stromal cells while maintaining substantial proliferative capacity during manufacturing.

01

Osteogenic

02

Chondrogenic

03

Adipogenic

Therapeutic Development Is Not Based on Tissue Replacement

Although differentiation capabilities help confirm MSC identity, current therapeutic development is not based on direct tissue replacement.

Instead, accumulating evidence indicates that biological activity is primarily associated with secreted mediators and immunomodulatory signaling.

Cytokines Growth Factors Chemokines Exosomes Immunomodulatory Signaling Molecules
Manufacturing

Designed for Large-Scale Allogeneic Manufacturing

One of JadiCell's distinguishing characteristics is its potential suitability for large-scale allogeneic manufacturing.

Beginning with carefully screened umbilical cord tissue donors, cells are expanded under controlled Good Manufacturing Practice (GMP) conditions to generate standardized clinical product.

The allogeneic manufacturing model has the potential to generate numerous standardized treatment doses from a qualified donor-derived starting population, supporting scalability while avoiding patient-specific manufacturing.

Manufacturing Workflow

From Qualified Donor Tissue to Clinical Distribution

A controlled manufacturing pathway designed to support reproducibility, product characterization, quality control, cryopreservation, and scalable distribution.

01
Donor Qualification Qualified Starting Material
02
Umbilical Cord Tissue Procurement Controlled Tissue Source
03
Primary Cell Derivation Subepithelial Tissue Isolation
04
Master Cell Bank MCB
05
Working Cell Bank WCB
06
Controlled Expansion Process-Controlled Scale-Up
07
Release Testing Identity · Quality · Safety
08
Cryopreservation Standardized Clinical Product
09
Clinical Distribution Allogeneic · Off-the-Shelf
Quality Control

Product Characterization & Release Testing

Manufacturing incorporates extensive release testing intended to support product identity, quality, safety, reproducibility, and suitability for clinical use.

Cell Identity
Viability
Sterility
Endotoxin
Mycoplasma
Purity
Morphology
Potency Assays
Genetic Stability
Cryopreservation Validation
Manufacturing Characteristics

Supporting an Allogeneic Development Model

Published laboratory work has reported manufacturing characteristics relevant to continued development and potential scalability of the JadiCell™ platform.

01

Proliferative Capacity

Published work has reported extended proliferative capacity, including more than 90 population doublings under reported laboratory conditions.

02

Stable Morphology

Reported studies describe maintenance of characteristic cellular morphology during prolonged culture and expansion.

03

Genetic Stability

Published laboratory studies have reported maintenance of a normal chromosomal karyotype during prolonged culture under the reported experimental conditions.

Scalability

One Qualified Starting Population, Multiple Standardized Doses

The allogeneic manufacturing model has the potential to generate numerous standardized clinical doses from a qualified donor-derived starting population.

This model is intended to support scalability while avoiding the individualized manufacturing requirements associated with patient-specific autologous cellular therapies.

Manufacturing qualification: This section describes the general manufacturing framework and published characteristics associated with the JadiCell™ platform. Final clinical manufacturing processes, specifications, assays, acceptance criteria, release requirements, and product controls are governed by applicable controlled manufacturing and regulatory documentation.

Intellectual Property

Proprietary Platform Protection

JadiCell is supported by a proprietary intellectual property portfolio centered on U.S. Patent No. 9,803,176 B2.

The patent describes mesenchymal cells isolated from the subepithelial layer of umbilical cord tissue, along with methods for their isolation, expansion, characterization, and therapeutic use.

The intellectual property differentiates JadiCell from many MSC platforms that utilize alternative tissue sources such as bone marrow, adipose tissue, Wharton's jelly, placenta, dental pulp, or peripheral blood.

Proprietary Tissue Source
Isolation Methodology
Cell Characterization
Surface-Marker Profile
Expansion Methods
Manufacturing Processes
Cryopreservation
Therapeutic Applications

Important: Patent protection does not establish clinical efficacy or regulatory approval. Clinical benefit must ultimately be demonstrated through adequately controlled clinical trials.